추천 제품
제품 라인
BioReagent
형태
powder
기술
cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable
EEO
≤0.1
mp
≤65 °C ( at a 1.5% gel)
전이 온도
congealing temperature 26-30 °C
겔 강도
≥200 g/cm2 (1% gel)
solubility
H2O: 10 mg/mL (with heat)
음이온 미량물
sulfate (SO42-): ≤0.10%
외래 활성
RNase and DNase free
일반 설명
Agarose is an algal polysaccharide. Agarose is a thermoreversible, ion-dependent gelling agent. Agarose gel electrophoresis is useful for the clinical routine analyses of proteins in plasma and other body fluids. It is a low gelling temperature derivative with unique gelling properties. Gels forms at <30°C, remelt at temperatures in excess of 60°C. Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials.
애플리케이션
Agarose, a low gelling temperature derivative, is the suitable reagent for:
It may be used for the following studies:
- plant cell culture studies
- insect cell culture studies
- cell culture studies
It may be used for the following studies:
- Recovery of defined RNA and DNA fractions after electrophoretic separation.
- Cytochemical staining procedure to investigate the succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes.
- Purification of RNA in Caenorhabditis elegans by electrophoresis.
분석 메모
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Agarose gel electrophoresis.
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