E9379

Stains-All

~95% purity, powder

• 동의어(들): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• 실험식(Hill 표기법): C₃₀H₂₇BrN₂S₂
• CAS Number: 7423-31-6
• Molecular Weight: 559.58
• Beilstein: 3865213
• EC Number: 231-047-7
• MDL number: MFCD00078788
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24894694
• NACRES: NA.47

속성

• 제품명: Stains-All, ~95%
• 분석: ~95%
• Quality Level: 200
• 양식: powder
• 기술: microbe id | staining: suitable
• solubility: chloroform: 40 mg (PLUS 4.0 ml)
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: MPBRYMWMMKKRGC-UHFFFAOYSA-M

설명

애플리케이션

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

생화학적/생리학적 작용

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

주의사항

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)