N6876

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

• 동의어(들): 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
• Linear Formula: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS Number: 298-83-9
• Molecular Weight: 817.64
• Beilstein: 4115923
• MDL number: MFCD00012159
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24897795
• NACRES: NA.47

속성

• Quality Level: 200
• 분석: ≥90.0% (HPLC)
• 형태: powder
• 불순물: ≤10% solvent
• solubility: H₂O: 10 mg/mL
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: 2-8°C
• SMILES string: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChI key: FSVCQIDHPKZJSO-UHFFFAOYSA-L

설명

애플리케이션

• Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
• It has been used as a substrate for western blot detection.
• It has been used for the staining of histological tissue sections.

생화학적/생리학적 작용

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

기질

Substrate for dehydrogenases and other oxidases.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302,H315,H319,H335,H371
• 예방조치 성명서: P260 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P311
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3
• 표적 기관: Eyes,Central nervous system, Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable