PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• 동의어(들): PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• UNSPSC 코드: 12161700
• NACRES: NA.21

속성

• product name: Phosphate Buffered Saline with 0.05% TWEEN^® 20, pH 7.4, pHast Pack^™, powder
• 제품 라인: pHast Pack^™
• 형태: powder
• 저장 조건: (Tightly closed. Dry)
• 불순물: ≤5 ppm Heavy Metals (lead)
• 색상: white
• pH: 7.2—7.6 (25 °C)
• solubility: soluble, clear to hazy, colorless
• 양이온 미량물: Fe: <10 ppm
• 외래 활성: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 저장 온도: room temp

설명

일반 설명

Phosphate Buffered Saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween^® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

애플리케이션

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

특징 및 장점

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

포장

Foil pouches

재구성

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

저장 및 안정성

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable