PPB006

Phosphate buffered saline

powder, pH 7.4, pHast Pack^™

• 동의어(들): 1X PBS wash buffer, PBS
• MDL number: MFCD00131855
• UNSPSC 코드: 12161700
• NACRES: NA.21

속성

• 제품명: Phosphate buffered saline, pH 7.4, pHast Pack^™, powder
• 제품 라인: pHast Pack^™
• 양식: powder
• 저장 조건: (Tightly closed. Dry)
• 불순물: <5—2 ppm Heavy metals (Lead)
• 색상: white
• pH: 7.4
• solubility: soluble, clear, colorless
• 양이온 미량물: Fe: <10 ppm
• 외래 활성: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• 저장 온도: room temp

설명

일반 설명

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

애플리케이션

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

특징 및 장점

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

포장

Foil pouches

재구성

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

저장 및 안정성

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable