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일반 설명
Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.
애플리케이션
Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.
특징 및 장점
Cell Counting Kit has the following advantages:
- It doesn’t require any radioisotopes to function.
- No solubilization procedure is needed.
- Results are highly reproducible and accurate.
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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