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Merck
모든 사진(1)

주요 문서

04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

동의어(들):

Staining kit for live/dead cells

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.32

가격 및 재고 정보를 현재 이용할 수 없음

적합성

suitable for fluorescence

Quality Level

저장 온도

−20°C

애플리케이션

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

키트 구성품 전용

제품 번호
설명

  • Solution A (Calcein AM solution) 4 × 50

  • Solution B (propidium iodide solution) 300 μL

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

185.0 °F - closed cup

Flash Point (°C)

85 °C - closed cup


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시험 성적서(COA)

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문서 라이브러리 방문

T Kimura et al.
Neuroscience letters, 208(1), 53-56 (1996-04-12)
The recent immunological demonstration of advanced glycation end products (AGE) of the Maillard reaction in several human tissues suggests a possible involvement of AGE in the aging process. We previously prepared a monoclonal anti-AGE antibody (6D12) which recognized N epsilon-(carboxymethyl)lysine.
Amin Hassanzadeh-Barforoushi et al.
Lab on a chip, 18(15), 2156-2166 (2018-06-21)
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized
Camille Raillon et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(10), 1085-1095 (2019-08-01)
The isolation, analysis, and enumeration of circulating tumor cells (CTCs) from cancer patient blood samples are a paradigm shift for cancer patient diagnosis, prognosis, and treatment monitoring. Most methods used to isolate and enumerate these target cells rely on the
Carolina Fracalossi Rediguieri et al.
Journal of biomaterials science. Polymer edition, 28(16), 1918-1934 (2017-07-25)
The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in
Rossella Barenghi et al.
BioMed research international, 2014, 624645-624645 (2014-11-19)
One of the main open issues in modern vascular surgery is the nonbiodegradability of implants used for stent interventions, which can lead to small caliber-related thrombosis and neointimal hyperplasia. Some new, resorbable polymeric materials have been proposed to substitute traditional

문서

암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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