MilliporeSigma
All Photos(1)

Documents

77730

Millipore

Gram Staining Kit

for microscopy, used for differentiation of bacteria on the basis of their gram nature.

Sign Into View Organizational & Contract Pricing

Synonym(s):
Bacteria Staining Kit according to Gram
NACRES:
NA.85

grade

for microscopy

Quality Level

Agency

according to GB 4789.30-2016

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical

suitability

bacteria

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
62650NA2120NA2110
Millipore

Millipore

77730

Gram Staining Kit

Lugol solution for microscopy (Bact., Bot.)

Sigma-Aldrich

62650

Lugol solution

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

grade

for microscopy

grade

for microscopy (Bact., Bot.)

grade

-

grade

-

agency

according to GB 4789.30-2016

agency

-

agency

-

agency

-

product line

BioChemika

product line

-

product line

-

product line

-

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

General description

The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red

Application

The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.

Components

Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Potential Threapeutic Applications of Extract Made from Electroporated Xenopus
Laevis frog Oocytes in Murine Models of Melanoma, Traumatic Brain Injury and
Experimental Skin Wrinkling
Sergei Paylian
Bjog : An International Journal of Obstetrics And Gynaecology (2016)
Bacterial colonization of winter flounder Pseudopleuronectes
americanus fed live feed enriched with three different commercial diets
L. H. Seychelles
Aquaculture Nutrition (2011)
Intracoronary allogeneic cardiosphere-derived stem cells are safe for use in dogs with dilated cardiomyopathy
Michael T
Journal of Cellular and Molecular Medicine (2017)
U E Rodríguez-Castrejón et al.
Archives of microbiology, 204(3), 191-191 (2022-02-24)
The study of arsenic (As)-resistant microorganisms with high As removal capacity is fundamental for the development of economically sustainable technologies used for the treatment of water contaminated with metalloid. In the current study, four bacterial strains were isolated from As-contaminated
Prevalence of blaOXA-?-Lactamase-Producing and Methicillin-Resistant Staphylococcus aureus in Iran
Navidinia M
AANA Journal (2016)

Articles

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens can cause contamination in undercooked or improperly sterillized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Campylobacter grows only on complex media that have been amended with diverse essential amino acids and supplements. We offer a broad range of specific agars and broths for the detection, identification, and cultivation of Campylobacter.

See All

Protocols

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service