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90107

Sigma-Aldrich

Gram′s iodine solution

for microscopy

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Synonym(s):
Potassium Triiodide
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

100

product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

color

brown to very dark brown

refractive index

n20/D 1.335

density

1.005 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

bacteria

Related Categories

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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