52583

Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast

≥2000 U/g

• 동의어(들): Candida antarctica Lipase
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in yeast
• Quality Level: 100
• 양식: beads
• 특이 활성도: ≥2000 U/g
• 분자량: 33 kDa
• 저장 온도: 2-8°C

설명

일반 설명

Lipase B or triacylglycerol ester hydrolases belongs to the class of hydrolases. Lipase B from Candida antarctica (CALB) possesses 317 amino acids and a molecular weight of 33 kDa. CALB has a catalytic triad and an open solvent accessible active site. The catalytic triad is usually Ser-His-Asp/Glu which is found in the carboxy terminal of parallel β sheet.

애플리케이션

Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast has been used:

• in esterification reaction of lauric acid with n-butanol in a biphasic solvent system
• in hydrolysis of fish oil triglycerides
• in screening of enzymes for Morita–Baylis–Hillman (MBH) reaction

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

생화학적/생리학적 작용

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Lipases B from Candida antarctica (CALB) is a versatile catalyst for biotransformation reactions. In aqueous media, CALB behaves like an esterase rather than a lipase.

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

단위 정의

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)