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Merck
모든 사진(1)

주요 문서

73737

Sigma-Aldrich

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

동의어(들):

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
Molecular Weight:
461.36
Beilstein:
1578142
EC Number:
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.32

제품명

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Quality Level

분석

≥97.0% (enzymatic)

양식

powder or crystals

품질

for the determination of phosphatase

불순물

≤0.1% free nitrophenol
≤6% water

solubility

H2O: 0.1 g/mL, clear

저장 온도

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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애플리케이션

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

생화학적/생리학적 작용

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Ulrike Lorenz
Current protocols in immunology, Chapter 11, Unit 11-Unit 11 (2011-04-05)
Tyrosine phosphorylation and dephosphorylation of proteins play a critical role in many processes of the immune system, from early development to fully differentiated effector function. Since the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) determine
Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
O.H. Lowry
Methods in Enzymology, 4, 366-366 (1957)

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