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Merck
모든 사진(1)

주요 문서

A0412

Sigma-Aldrich

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

affinity isolated antibody

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

Quality Level

결합

peroxidase conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

종 반응성

mouse

기술

direct ELISA: 1:10,000 using IgG, IgA, IgM

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria, and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells,,. Goat anti-mouse polyvalent immunoglobulins (G,A,M)-peroxidase antibody binds to mouse IgG, IgA and IgM.

애플리케이션

The detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Relative binding affinities for specific Mabs were determined by an inhibition ELISA using peroxidase-conjugated goat anti-mouse polyvalent antibody diluted 1:10,000 in PBST and incubated for 1 hour at 21-23°C.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

제조 메모

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Nicole Forster et al.
Journal of immunology (Baltimore, Md. : 1950), 178(11), 6941-6948 (2007-05-22)
Conditional knock-in mice expressing a histone acetyltransferase-deficient version of the transcriptional coregulator p300 exclusively in B lymphocytes die prematurely with full penetrance. The mice develop an autoimmune disease similar to systemic lupus erythematosus in its pathological manifestations, such as splenomegaly
M Chen et al.
Infection and immunity, 68(9), 4893-4899 (2000-08-19)
Formation of anti-Toxoplasma gondii HSP70 (TgHSP70) antibody cross-reactive to mouse HSP70 (mHSP70) was observed in the sera of BALB/c (a resistant strain) and C57BL/6 (B6; a susceptible strain) mice after peroral infection with T. gondii cysts of the Fukaya strain.
Hong Xin et al.
Infection and immunity, 74(7), 4310-4321 (2006-06-23)
We previously reported the enhanced resistance of monoclonal antibodies B6.1 (an immunoglobulin M [IgM]) and C3.1 (an IgG3) against experimental candidiasis. Both MAbs recognize the same fungal epitope. We have since found that a highly passaged B6.1 hybridoma (hp-B6.1) resulted
The Effect of the Herbicide Mecoprop on Heliscus lugdunensis and Its Influence on the Preferential Feeding of Gammarus Pseudolimnaeus.
S Bermingham et al.
Microbial ecology, 35(2), 199-204 (1998-12-16)
Sze M Lo et al.
Renal failure, 38(4), 558-563 (2016-02-26)
Chronic kidney disease (CKD) progression and complications are associated with increased oxidative stress, as well as with Nrf2 inactivation. Lipoic acid (LA) has been considered an inducer of Nrf2 antioxidant response. We tested whether oral administration of LA provides beneficial

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