추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
AC-15, monoclonal
양식
buffered aqueous solution
분자량
antigen 42 kDa
종 반응성
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
반응하면 안 됨
Dictyostelium discoideum
기술
immunocytochemistry: 10-40 μg/mL using human foreskin fibroblasts
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.
동형
IgG1
UniProt 수납 번호
응용 분야
research pathology
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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일반 설명
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
The ACTB (β-actin) gene is mapped to human chromosome 7p22.1. β-Actin is the most abundant protein localized to the cytoplasm. ACTB is expressed ubiquitously. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds. Four of the actin isoforms represent the differentiation markers of muscle tissues and two are found in almost all cells. Anti-β-Actin antibody, Mouse Monoclonal (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a slightly modified synthetic b-cytoplasmic actin N-terminal peptide Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
특이성
Anti-β-Actin antibody, Mouse Monoclonal 1, 2 recognizes an epitope located on the N-terminal end of the β-isoform of actin.
면역원
slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
애플리케이션
Monoclonal anti-beta-actin antibody can be used for microarray, indirect immunofluorescence, and immunohistochemical analyses. Furthermore, the product has been used for immunocytochemistry at 10-40 μg/mL using human foreskin fibroblasts. The antibody has also been used for western blot at 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
The actin in cells of various species and tissue origin is very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera to this protein. Therefore the availability of monoclonal antibodies to β-actin provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Monoclonal anti-beta-actin antibody can be used for microarray, indirect immunofluorescence, and immunohistochemical analyses. Furthermore, the product has been used for immunocytochemistry at 10-40 μg/mL using human foreskin fibroblasts. The antibody has also been used for western blot at 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding.
It has been used in Immunoblot analysis.
Monoclonal anti-beta-actin antibody can be used for microarray, indirect immunofluorescence, and immunohistochemical analyses. Furthermore, the product has been used for immunocytochemistry at 10-40 μg/mL using human foreskin fibroblasts. The antibody has also been used for western blot at 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding.
It has been used in Immunoblot analysis.
생화학적/생리학적 작용
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.
ACTB (β-actin) is crucial for organogenesis, especially for the development of brain, kidney and heart. It is responsible for maintaining cell proliferation, migration and shape. Actin protein is essential for the formation of mature platelets. β−Actin plays a key role in embryonic development. Upregulation of β-actin stimulates membrane protrusions and helps in cellular motility. The encoded protein β-Actin mediates fibroblast migration in mouse and thereby control connective tissue tension.
Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
ACTB (β-actin) is crucial for organogenesis, especially for the development of brain, kidney and heart. It is responsible for maintaining cell proliferation, migration and shape. Actin protein is essential for the formation of mature platelets. β−Actin plays a key role in embryonic development. Upregulation of β-actin stimulates membrane protrusions and helps in cellular motility. The encoded protein β-Actin mediates fibroblast migration in mouse and thereby control connective tissue tension.
Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
제조 메모
Storage and Stability: For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
기타 정보
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
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문서
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
프로토콜
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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