추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
클론
monoclonal
형태
liquid
특징
hotstart
농도
1.1 mg/mL
기술
PCR: suitable
색상
colorless
배송 상태
wet ice
저장 온도
−20°C
일반 설명
JumpStart™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.
애플리케이션
JumpStart™Taq Antibody is suitable for use in:
- cytochrome blocked nucleic acid (LNA) probe assay
- ligation-mediated polymerase chain reaction (LM-PCR)
- in Real-Time quantitative PCR Analysis of viral titer
- PCR amplification of complex genomic or cDNA templates
- to amplify very low-copy-number targets
- for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube
포장
Supplied with dilution buffer.
성분
JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials
Dilution Buffer provided in 1 mL vials
수량
Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.
기타 정보
JumpStart™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
법적 정보
JumpStart is a trademark of Sigma-Aldrich Co. LLC
관련 제품
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Phytopathology, 97(3), 297-303 (2007)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
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