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Merck
모든 사진(1)

주요 문서

A8011

Sigma-Aldrich

Antifoam C Emulsion

aqueous-silicone emulsion

동의어(들):

Antifoaming Agent Emulsion

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About This Item

UNSPSC 코드:
12161900
NACRES:
NA.25

생물학적 소스

synthetic

Quality Level

형태

emulsion

포함

emulsifier

기술

cell culture | hybridoma: suitable
microbiological culture: suitable

mp

0  °C (32 °F)

일반 설명

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

애플리케이션

Antifoam C emulsion has been used:
  • as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
  • as a component of feed medium,
  • as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
  • as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

특징 및 장점

  • Minimizes compatibility issues with biological systems
  • Highly effective defoamer at low concentrations
  • Highly effective and versatile silicone defoamer

기타 정보

A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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문서 라이브러리 방문

Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Magdalena Pappenreiter et al.
Frontiers in bioengineering and biotechnology, 7, 195-195 (2019-09-10)
In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be

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