추천 제품
생물학적 소스
bovine
Quality Level
Grade
for molecular biology
분석
≥98% (agarose gel electrophoresis)
형태
lyophilized powder
품질
deacetylated
분자량
~66 kDa
정제법
heat shock fractionation
포장
glass bottle of
기원
USA origin
기술
electrophoresis: suitable-98% using Agarose
불순물
enzymatic impurity, none detected
pH
7
solubility
water: soluble (50 mg/ml)
적합성
suitable for (for molecular biology)
UniProt 수납 번호
외래 활성
Alkaline Phosphatase free
Alkaline phosphatase and peroxidase, none detected
DNase free
DNase, RNase, and protease, none detected
Peroxidase free
Protease free
RNase free
저장 온도
2-8°C
유전자 정보
bovine ... ALB(280717)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Bovine Serum Albumin (BSA) is a plasma protein produced in the bovine liver that has a globular, non-glycosylated α-helical structure. It consists of a single polypeptide chain with 583 amino acid residues and 17 cysteine residues that form the cross-linked disulfide bridges, stabilizing its structure. The BSA protein comprises three structurally distinct domains (I, II, and III), each of which contains two sub-domains (A and B) arranged in a heart-shaped architecture.
애플리케이션
Bovine Serum Albumin (BSA) has been used
- as a component of phosphate buffer saline (PBS) solution for dissolving tumor necrosis factor α (TNFα), which was added as a supplement for culture media
- as a blocking agent to reduce non-specific binding during the albumin sensor assay
- as a blocking agent in the immunofluorescence (IF) assay
- as a protein standard in the Bradford assay
생화학적/생리학적 작용
Bovine Serum Albumin (BSA) is a versatile protein commonly utilized in cell and tissue culture media as a supplement. It helps in lipid solubilization and stabilizing other proteins. BSA is also a carrier for small molecules such as steroids, fatty acids, and thyroid hormones. It serves as a standard protein for immunochemical tests to determine protein concentration in a solution and calibration studies.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
특징 및 장점
This BSA product for molecular biology is tested for the following properties:
* Alkaline Phosphatase-free
* DNase-free / Exonuclease-free
* Nickase-free / Endonuclease-free
* Peroxidase-free
* Protease-free
* RNase-free
* Alkaline Phosphatase-free
* DNase-free / Exonuclease-free
* Nickase-free / Endonuclease-free
* Peroxidase-free
* Protease-free
* RNase-free
제조 메모
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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