C1345

Catalase from bovine liver

powder, suitable for cell culture, 2,000-5,000 units/mg protein

• 동의어(들): H₂O₂:H₂O₂ oxidoreductase
• CAS Number: 9001-05-2
• 효소 위원회 번호: 1.11.1.6 (BRENDA, IUBMB)
• EC Number: 232-577-1
• MDL number: MFCD00081483
• UNSPSC 코드: 12352204
• eCl@ss: 32160410
• NACRES: NA.75

속성

• 생물학적 소스: bovine liver
• Quality Level: 200
• 제품 라인: BioReagent
• 양식: powder
• 특이 활성도: 2,000-5,000 units/mg protein
• 분자량: tetramer ~250 kDa
• 기술: cell culture | mammalian: suitable
• 등전점: 5.4
• UniProt 수납 번호: P00432
• 배송 상태: wet ice

설명

일반 설명

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.[1]

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

애플리케이션

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Catalase from bovine liver is used for the following applications:

• Gel filtration[2]
• Analytical filtration on Ultrogel and glycerol gradient centrifugation[3]
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor[4]
• Enzyme assays[5][6]
• Alcohol oxidase activity[7]

생화학적/생리학적 작용

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

주의사항

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

단위 정의

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

제조 메모

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)