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Merck
모든 사진(2)

주요 문서

C3777

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type II, essentially salt-free, lyophilized powder

동의어(들):

FITC-casein

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About This Item

MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32

유형

Type II

Quality Level

양식

essentially salt-free, lyophilized powder

라벨링 범위

20-50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy, yellow to orange

저장 온도

2-8°C

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일반 설명

Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, αS1, αS2, β, and κ. Casein exists as micelles in milk.

애플리케이션

Casein fluorescein isothiocyanate from bovine milk has been used:
  • as a substrate for semi-quantitative analysis of protease in Arabidopsis cells
  • to determine the caseinolytic activity of secreted LasB (elastase), alkaline protease and protease IV
  • for determining the SpeB (cysteine protease) proteolytic activity in Streptococcus pyogenes cells

highly sensitive protease substrate

생화학적/생리학적 작용

Casein micelle system is involved in the prevention of pathological calcification of mammary glands.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Invited review: Understanding the behavior of caseins in milk concentrates
Corredig M, et al.
Journal of Dairy Science, 102(6), 4772-4782 (2019)
Casein and Whey Proteins in Human Health
Milk and Dairy Products as Functional Foods, 102(6), 94-146 (2014)
Cationic antimicrobial peptides disrupt the Streptococcus pyogenes ExPortal
Vega LA and Caparon MG
Molecular Microbiology, 85(6), 1119-1132 (2012)
Thomas S Murray et al.
Journal of medical microbiology, 59(Pt 5), 511-520 (2010-01-23)
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen capable of acutely infecting or persistently colonizing susceptible hosts. P. aeruginosa colonizes surfaces in vitro by either biofilm formation or swarming motility. The choice of behaviour is influenced by the physical properties of
Laura Reinhardt et al.
mBio, 13(6), e0141322-e0141322 (2022-10-27)
Clp proteases consist of a proteolytic, tetradecameric ClpP core and AAA+ Clp-ATPases. Streptomycetes, producers of a plethora of secondary metabolites, encode up to five different ClpP homologs, and the composition of their unusually complex Clp protease machinery has remained unsolved.

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