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Merck
모든 사진(1)

주요 문서

SH-SY5Y Cell Line human

94030304, human nerve, Neuroblast

동의어(들):

SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells

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About This Item

UNSPSC 코드:
41106514

제품명

SH-SY5Y Cell Line human, 94030304, Neuroblast from neural tissue.

생물학적 소스

human nerve

성장 모드

Adherent

핵형

Not specified

형태학

Neuroblast

제품

Not specified

수용체

Not specified

기술

cell culture | mammalian: suitable

관련 질환(들)

metastasis

배송 상태

dry ice

저장 온도

−196°C

세포주 기원

Human neuroblastoma

세포주 설명

SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.

애플리케이션

SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
  • study the iron-mediated toxicity of amyloid β peptide
  • check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells
  • study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells

기타 정보

  • DNA is stored at -70 °C
  • RNA is stored at -70 °C
  • Cell line is stored at -196 °C
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DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

배양 배지

Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.

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시험 성적서(COA)

Lot/Batch Number

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