추천 제품
제품명
SH-SY5Y Cell Line human, 94030304, Neuroblast from neural tissue.
생물학적 소스
human nerve
성장 모드
Adherent
핵형
Not specified
형태학
Neuroblast
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
metastasis
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human neuroblastoma
세포주 설명
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
애플리케이션
SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
SH-SY5Y cell line human has been used to:
- study the iron-mediated toxicity of amyloid β peptide
- check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells
- study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells
기타 정보
- DNA is stored at -70 °C
- RNA is stored at -70 °C
- Cell line is stored at -196 °C
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DNA 프로파일
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
배양 배지
Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
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