추천 제품
일반 설명
The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.
애플리케이션
Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.
생화학적/생리학적 작용
Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.
Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues
특징 및 장점
- A quick and simple method
- The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
- The kit includes a deproteination reagent for biological samples
키트 구성품 전용
제품 번호
설명
- Assay Buffer 5x 30 mL
- DTNB 8 mg
- DMSO 7.5 mL
- Glutathine Reductase 20 U
- L-Glutathione Reduced 300 μg
- NADPH 25 mg
- 5-Sulfosalicylic Acid 2.5 g
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Maria Swastika et al.
Malaria journal, 19(1), 208-208 (2020-06-20)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme disorder in the world. Its main function is to generate NADPH that is required for anti-oxidative pathway in the cells especially in red blood cells (RBC). G6PD deficiency is X-linked and
Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E
Al-Damegh MA
Clinics, 67(7), 785-792 (2012)
Genotoxicity of hydroquinone in A549 cells
Peng C, et al.
Cell Biology and Toxicology, 29(4), 213-227 (2013)
Nicol M Caplin et al.
Frontiers in plant science, 11, 389-389 (2020-05-01)
The effects of ionising radiation (IR) on plants are important for environmental protection but also in agriculture, horticulture, space science, and plant stress biology. Much current understanding of the effects of IR on plants derives from acute high-dose studies but
Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples.
T P Akerboom et al.
Methods in enzymology, 77, 373-382 (1981-01-01)
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