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Merck
모든 사진(2)

주요 문서

CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

동의어(들):

Beta-NAG Activity Assay Kit

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About This Item

EC Number:
UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)

Quality Level

배송 상태

wet ice

저장 온도

2-8°C

유전자 정보

human ... NAGLU(4669)

애플리케이션

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

생화학적/생리학적 작용

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

분석 메모

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

키트 구성품 전용

제품 번호
설명

  • Dilution Buffer 8 mL

  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg

  • Citrate Buffer Solution, 0.09 M 100 mL

  • p-Nitrophenol Standard Solution, 10 mM 1 mL

  • β-N-Acetylglucosaminidase from Jack beans 1 vial

  • Sodium carbonate 5 g

관련 제품

제품 번호
설명
가격

픽토그램

Health hazardExclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

표적 기관

Liver,Kidney

Storage Class Code

11 - Combustible Solids


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시험 성적서(COA)

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문서

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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