추천 제품
사용
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
Quality Level
배송 상태
wet ice
저장 온도
2-8°C
유전자 정보
human ... NAGLU(4669)
애플리케이션
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
생화학적/생리학적 작용
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
분석 메모
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
키트 구성품 전용
제품 번호
설명
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
관련 제품
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
표적 기관
Liver,Kidney
Storage Class Code
11 - Combustible Solids
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
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문서
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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