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Merck
모든 사진(1)

주요 문서

GL0010

Sigma-Aldrich

Golgi Isolation Kit

sufficient for 50 g (tissue)

동의어(들):

Golgi Kit, Isolation Kit for Golgi

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

사용

sufficient for 50 g (tissue)

Quality Level

기술

fractionation: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

애플리케이션

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

분석 메모

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

V J Allan et al.
The Journal of cell biology, 113(2), 347-359 (1991-04-01)
When higher eukaryotic cells enter mitosis, membrane organization changes dramatically and traffic between membrane compartments is inhibited. Since membrane transport along microtubules is involved in secretion, endocytosis, and the positioning of organelles during interphase, we have explored whether the mitotic
E R Sjoberg et al.
The Journal of biological chemistry, 268(14), 10185-10196 (1993-05-15)
The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized. However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure. Here, we have studied the spatial and temporal interrelationships among the
Julien Villeneuve et al.
The Journal of cell biology, 217(2), 649-665 (2017-12-08)
An appreciation of the functional properties of the cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with the recent demonstration that an extracellular form secreted by adipocytes regulates a wide range of physiological functions. Little, however, is known about
A Surroca et al.
The Journal of membrane biology, 177(3), 243-249 (2000-10-03)
We investigated the direct effect of inositol 1,4,5-trisphosphate (IP(3)) and ryanodine receptor agonists on Ca(2+) release from vesicles of a rat liver Golgi apparatus (GA) enriched fraction, which were actively loaded with (45)Ca(2+). Results in GA were compared with those
E Prchla et al.
The Journal of cell biology, 131(1), 111-123 (1995-10-01)
Endosomal penetration by nonenveloped viruses might be accomplished by either local breakdown of the endosomal membrane (e.g., adenovirus) or formation of a membrane-spanning pore by capsid proteins. Uncoating of the nonenveloped virus human rhinovirus serotype 2 (HRV2) has been shown

문서

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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