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Merck
모든 사진(1)

문서

D1556

Sigma-Aldrich

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

동의어(들):

Iodixanol solution

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352207
PubChem Substance ID:
NACRES:
NA.75

무균

sterile

형태

liquid

density

1.319-1.321 g/mL

응용 분야

hematology
histology

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

일반 설명

OptiPrep Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.

애플리케이션

OptiPrep has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.
  • the gradient-based isolation of senescent cancer cells.
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

특징 및 장점

OptiPrep has numerous biocompatible properties that make it ideal for use.
  • It is biologically inert and does not interfere with assays for marker enzymes.
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.

기타 정보

60% (w/v) solution of iodixanol in water (sterile).

법적 정보

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Emerging Infectious Diseases, 21(1), 103-106 (2015)
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Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation
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