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Merck
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Key Documents

D8388

Sigma-Aldrich

Dimethyl pimelimidate dihydrochloride

powder

동의어(들):

DMP, Dimethyl pimelinediimidate dihydrochloride

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About This Item

Linear Formula:
[CH3OC(=NH)CH2CH2]2CH2 · 2HCl
CAS Number:
Molecular Weight:
259.17
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.25

형태

powder

반응 적합성

reagent type: cross-linking reagent

solubility

H2O: 10 mg/mL

저장 온도

−20°C

SMILES string

Cl.Cl.COC(=N)CCCCCC(=N)OC

InChI

1S/C9H18N2O2.2ClH/c1-12-8(10)6-4-3-5-7-9(11)13-2;;/h10-11H,3-7H2,1-2H3;2*1H

InChI key

LRHXBHUTQWIZTN-UHFFFAOYSA-N

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일반 설명

Dimethyl pimelimidate dihydrochloride is a homobifunctional cross-linking reagent used in crosslinking, protein interaction and protein modification. It typically reacts with primary amines in the pH range 7.0-10.0 to form amidine bonds.

애플리케이션

Dimethyl pimelimidate dihydrochloride has been used to develop a technique for isolating Enterocytozoon bieneusi spores directly from human stool samples with a high degree of purity, which opens up new approaches for studying this parasite.

주의사항

The reagent is readily hydrolyzed at neutral pH.

기타 정보

Note that the amidine linkage preserves original primary amine positive charge. Incorporates a seven atom linker.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Kevin Michael Boehm et al.
BMC bioinformatics, 20(1), 7-7 (2019-01-07)
To further our understanding of immunopeptidomics, improved tools are needed to identify peptides presented by major histocompatibility complex class I (MHC-I). Many existing tools are limited by their reliance upon chemical affinity data, which is less biologically relevant than sampling
G B Karlsson et al.
Analytical biochemistry, 199(2), 219-222 (1991-12-01)
A method is described for the use of magnetic beads as a solid phase for the immunoprecipitation of labeled proteins. The anti-human transferrin receptor monoclonal antibody OKT-9 has been coupled to sheep anti-mouse IgG1-coated magnetic beads using the crosslinking agent
I Accoceberry et al.
Journal of clinical microbiology, 39(5), 1947-1951 (2001-04-28)
An original, reliable, and reproducible method for the purification of Enterocytozoon bieneusi spores from human stools is described. We recently reported the production of a species-specific monoclonal antibody (MAb) 6E52D9 immunoglobulin G2a (IgG2a) raised against the exospore of E. bieneusi
Semisynthetic fluorohydrolases prepared by chemical modification of ribonuclease.
David, E., et al.
Enz. Microbiol. Technol., 14, 885-892 (1992)
K Brew et al.
The Journal of biological chemistry, 250(4), 1434-1444 (1975-02-25)
The cross-linking of the two components of lactose synthetase, alpha-lactalbumin and a galactosyltransferase, with dimethylpimelimidate was examined. The extent of the cross-linking at pH 8.1 was found to be dependent upon the presence of substrates or inhibitors for the galactosyltransferase.

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