D9655

pBR322 Hae III Digest

for DNA electrophoresis

• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• Quality Level: 100
• 양식: liquid
• 사용: 1000 uses (0.5 mg size); 200 uses (0.1 mg size)
• 적합성: suitable for electrophoresis (DNA)
• 저장 온도: −20°C

설명

일반 설명

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

애플리케이션

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

성분

Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

기타 정보

Contains 22 fragments, 8–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5.

제조 메모

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable