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Merck
모든 사진(2)

주요 문서

E2886

Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

동의어(들):

ExtrAvidin HRP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

결합

peroxidase conjugate

Quality Level

양식

buffered aqueous solution

기술

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

배송 상태

wet ice

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

특이성

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

애플리케이션

ExtrAvidin®-Peroxidase is suitable for the following applications:
  • Dot blot at a minimum dilution of 1:4,000
  • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
  • Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
  • Immunoprecipitation and Immunobloting
  • Ra2I solid-phase binding assays
  • Southwestern blotting
  • Western blotting
  • Immunocytochemistry

생화학적/생리학적 작용

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

제조 메모

Affinity purified protein

저장 및 안정성

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

법적 정보

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

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문서 라이브러리 방문

D Tuckwell et al.
Journal of cell science, 108 ( Pt 4), 1629-1637 (1995-04-01)
Integrins alpha 1 beta 1 and alpha 2 beta 1 are major cellular receptors for collagens. The alpha 1 and alpha 2 subunits contain a approximately 200 amino acid inserted domain (I-domain) in their N-terminal region and, because of the
Stefan Lüth et al.
The Journal of clinical investigation, 118(10), 3403-3410 (2008-09-20)
Tregs are important mediators of immune tolerance to self antigens, and it has been suggested that Treg inactivation may cause autoimmune disease. Therefore, immunotherapy approaches that aim to restore or expand autoantigen-specific Treg activity might be beneficial for the treatment
Mohamed S Benleulmi et al.
Retrovirology, 14(1), 54-54 (2017-11-29)
Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase·viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved
H Kaur et al.
Scandinavian journal of immunology, 62(4), 361-370 (2005-10-29)
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine
Andrea N Moreno-Amaral et al.
Journal of leukocyte biology, 91(3), 469-474 (2011-12-15)
M-ficolin specificity for sialylated ligands prompted us to investigate its interactions with the main membrane sialoprotein of human neutrophils, CD43. rM-ficolin bound CD43 and prevented the access of anti-CD43 mAb. Moreover, rM-ficolin reacted exclusively with CD43 on Western blots of

프로토콜

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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