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Merck
모든 사진(2)

주요 문서

A7419

Sigma-Aldrich

Avidin–Peroxidase

buffered aqueous solution

동의어(들):

Avidin–HRP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

avidin from egg white
enzyme from horseradish

Quality Level

결합

peroxidase conjugate

양식

buffered aqueous solution

기술

indirect ELISA: 1:100,000
western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

애플리케이션

Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

제조 메모

Affinity purified protein.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Bianca Schneider et al.
Traffic (Copenhagen, Denmark), 8(3), 226-240 (2007-02-27)
Phagocytic compartments are specialized endocytic organelles and usually mature along the degradative pathway into phagolysosomes. The rare human pathogen Afipia felis localizes to a compartment that is different from canonical phagocytic compartments. Here, we present evidence that internalization of Afipia
Erica A Peterson et al.
Journal of cell science, 116(Pt 12), 2399-2408 (2003-05-02)
Cell-surface annexin 2 (A2) and its ligand p11 have been implicated in fibrinolysis because of their ability to accelerate tissue plasminogen activator (tPA)-mediated activation of plasminogen to plasmin. Because thrombin is a potent cell modulator obligately produced at the site
Akitoshi Hasegawa et al.
American journal of obstetrics and gynecology, 192(4), 1038-1043 (2005-04-23)
Lactoferrin, an iron-binding glycoprotein found in cervical mucus and amniotic fluid, plays a defensive role against mucosal infections. This study examined the effect of recombinant human lactoferrin on preterm delivery in a rabbit model. Anesthetized rabbits were randomly assigned to
Dominika Lukas et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(8), E1480-E1489 (2017-02-09)
TGF-β is an anti-inflammatory cytokine whose signaling is negatively controlled by Smad7. Previously, we established a role for Smad7 in the generation of autoreactive T cells; however, the function of Smad7 in dendritic cells (DCs) remains elusive. Here, we demonstrate
Norio Chihara et al.
Current protocols in immunology, 113, 3-3 (2016-04-03)
Regulatory T cell-mediated suppression serves as a pivotal mechanism of negative regulation of immune-mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that prevent excessive inflammatory responses and maintain immune tolerance. The

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