A9941

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

chromogenic, tablet

• 동의어(들): AzBTS-(NH₄)₂, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
• 실험식(Hill 표기법): C₁₈H₂₄N₆O₆S₄
• CAS Number: 30931-67-0
• Molecular Weight: 548.68
• Beilstein: 7329461
• EC Number: 250-396-6
• MDL number: MFCD00010404
• UNSPSC 코드: 12352204
• PubChem Substance ID: 24891410
• NACRES: NA.21

속성

• product name: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, tablet, 10 mg substrate per tablet
• Quality Level: 200
• 형태: tablet
• solubility: deionized water: 100 mL
• 저장 온도: room temp
• SMILES string: [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
• InChI: 1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
• InChI key: OHDRQQURAXLVGJ-AXMZSLBLSA-N

설명

일반 설명

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:

• For a reaction with laccase or bilirubin oxidase
• For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide

A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

애플리케이션

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
• ABTS is also used to measure glucose concentrations in different solutions including blood serum.
• The food industry uses ABTS to measure the antioxidant capacities within different foods.
• Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

재구성

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves