추천 제품
형태
solution (ready-to-use)
Quality Level
사용
sufficient for 1,500-3,000 assays (ELISA)
포장
pkg of 3 × 100 mL
제조업체/상표
Roche
기술
ELISA: suitable
저장 온도
2-8°C
일반 설명
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
애플리케이션
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
생화학적/생리학적 작용
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
물리적 형태
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
분석 메모
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
ABTS is a trademark of Roche
WGK
nwg
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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