추천 제품
일반 설명
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.
애플리케이션
ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
제조 메모
Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
ABTS is a trademark of Roche
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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