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Merck
모든 사진(2)

문서

11557

Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

동의어(들):

AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

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About This Item

실험식(Hill 표기법):
C18H24N6O6S4
CAS Number:
Molecular Weight:
548.68
Beilstein:
7329461
EC Number:
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

분석

≥98% (HPLC)

형태

powder or crystals

불순물

≤6.0% water

solubility

H2O: 10 mg/mL, slightly hazy to clear

저장 온도

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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애플리케이션

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:
  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts
  • to test the antioxidant capacity (AOC) of Aloe vera samples
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

기타 정보

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Antioxidant and cytotoxicological effects of Aloe vera food supplements
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A renewed interest in antioxidants has arisen in recent years; microalgae and cyanobacteria are potential sources thereof for use as food/feed ingredients. However, improved methods for comprehensive screening of antioxidant capacity specifically in intracellular extracts of marine microorganisms are required
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