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Merck
모든 사진(1)

문서

E5529

Millipore

EZview Red Streptavidin Affinity Gel

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

형태

suspension

Quality Level

유통기한

1 yr

기술

western blot: suitable

기질

4% agarose

pH

7.2

용량

~10 μg(biotin per ml of packed gel)

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.

애플리케이션

Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

특징 및 장점

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

물리적 형태

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

법적 정보

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Alicia M Bostwick et al.
Biochemical and biophysical research communications, 528(3), 580-585 (2020-06-09)
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria
Suet Yin Sarah Fung et al.
Current biology : CB, 27(1), 78-86 (2016-12-13)
After cleavage furrow ingression during cytokinesis, nascent daughter cells remain connected by an intercellular bridge (ICB) and the midbody [1, 2]. The midbody becomes an assembly platform for ESCRT complexes that split apart the plasma membrane (PM) anchored to the
Katarzyna M Zientara-Rytter et al.
Cells, 11(1) (2022-01-12)
Pex11, an abundant peroxisomal membrane protein (PMP), is required for division of peroxisomes and is robustly imported to peroxisomal membranes. We present a comprehensive analysis of how the Pichia pastoris Pex11 is recognized and chaperoned by Pex19, targeted to peroxisome
Ting-Yu Chang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111485-111485 (2021-03-20)
Aberrant alteration of epigenetic information disturbs chromatin structure and gene function, thereby facilitating cancer development. Several drugs targeting histone deacetylases (HDACs), a group of epigenetic enzymes, have been approved for treating hematologic malignancies in the clinic. However, patients who suffer
Iram Khan Iqbal et al.
Autophagy, 17(11), 3511-3529 (2021-01-19)
The deacetylase SIRT1 (sirtuin 1) has emerged as a major regulator of nucleocytoplasmic distribution of macroautophagy/autophagy marker MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Activation of SIRT1 leads to the deacetylation of LC3 and its translocation from the nucleus into

관련 콘텐츠

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

친화성 또는 GST 풀다운, 직렬 친화성 정제(TAP), 공동면역침전법을 사용하여 in vitro 단백질-단백질 상호작용을 연구하기 위한 풀다운 분석, 시약, 프로토콜.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.