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Merck
모든 사진(2)

문서

F8888

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

Quality Level

결합

FITC conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

형태

buffered aqueous solution

저장 조건

protect from light

기술

direct immunofluorescence: 1:320 using human peripheral blood lymphocytes
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.
Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

특이성

Binds chicken IgY (IgG). Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for chicken IgY (IgG).

면역원

Purified chicken IgY (IgG)
chicken IgY (IgG)

애플리케이션

Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody has been used in immunofluorescence.
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples.

생화학적/생리학적 작용

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens
Ingrao F, et al.
Avian Pathology : Journal of the W.V.P.A, 46(3), 289-299 (2017)
Alan Buckley et al.
The Journal of general virology, 84(Pt 10), 2807-2817 (2003-09-19)
The introduction and rapid dispersal of the African flavivirus West Nile virus (WNV) throughout North America, and the high fatality rate due to encephalitis in birds, horses, other wildlife species and humans, has attracted major attention worldwide. Usutu virus, another
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E Domínguez-Sala et al.
Brain structure & function, 227(5), 1599-1614 (2022-02-02)
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E J Thompson et al.
Neoplasia (New York, N.Y.), 3(5), 402-410 (2001-11-01)
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