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Merck
모든 사진(1)

주요 문서

G3042

Sigma-Aldrich

Anti-GAL4 DNA-BD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Galactose 4 DNA Binding Domain

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.56

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

기술

western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

특이성

Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.

면역원

synthetic peptide corresponding to the Saccharomyces cerevisiae GAL4 protein DNA binding domain (amino acids 39-52).

애플리케이션

Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.

생화학적/생리학적 작용

GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

저장 및 안정성

For continuous use store at 2-8°C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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