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Merck
모든 사진(2)

문서

ROAHAHA

Roche

Anti-HA High Affinity

from rat IgG1

동의어(들):

antibody

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About This Item

UNSPSC 코드:
12352203

생물학적 소스

rat

Quality Level

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

3F10, monoclonal

형태

lyophilized

포장

pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)

제조업체/상표

Roche

동형

IgG1

에피토프 서열

YPYDVPDYA

저장 온도

2-8°C

관련 카테고리

일반 설명

Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

특이성

Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

면역원

Amino acids 98-106 from the human influenza virus hemagglutinin protein

애플리케이션

Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
  • Dot blots
  • ELISA
  • Immunocytochemistry
  • Immunoprecipitation
  • Western blots
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

품질

Function tested in western blot.

제조 메모

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
  • Immunoprecipitation: 0.5 to 5 μg/ml
  • Western blot: 50 to 200 ng/ml

재구성

Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.

기타 정보

For life science research only. Not for use in diagnostic procedures.

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픽토그램

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신호어

Warning-Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

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