추천 제품
생물학적 소스
rat
Quality Level
결합
biotin conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3F10, monoclonal
양식
lyophilized (stabilized)
포장
pkg of 50 μg
제조업체/상표
Roche
동형
IgG1
에피토프 서열
YPYDVPDYA
저장 온도
2-8°C
관련 카테고리
일반 설명
Anti-HA-Biotin, High Affinity (3F10) is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to biotin. The Anti-HA-Biotin, High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5. It is a monoclonal antibody whose high affinity and low working concentrations result in less cross-reactivity than with other antibodies to the HA-epitope. Anti-HA-Biotin, High Affinity (3F10) is a biotin conjugate of this clone which is specifically useful in western blotting, ELISA applications and assays using the universal biotin-streptavidin platform, by allowing specific and highly sensitive detection of HA-tagged proteins.
특이성
Anti-HA-Biotin, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
면역원
Amino acids 98-106 from the human influenza virus hemagglutinin protein
애플리케이션
Anti-HA-Biotin, High Affinity (3F10) is used for the detection of HA-tagged recombinant proteins using:
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
- Dot blots
- ELISA (enzyme-linked immunosorbent assay)
- Western blots
It has also been used for immunocytochemistry, immunofluorescence and αScreen format based assay.
품질
Function test: The Anti-HA-Biotin; High Affinity is function tested by Western blot analysis of a HA-tagged fusion protein.
제조 메모
Sample Materials
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
Sample preparation: Prepare protein extracts containing the HA-tagged protein of interest using any of a variety of standard methods. The following lysis buffers have performed well and should be taken as guidelines:
- Bacterial extracts: 20 mM Tris, pH 8.0, 100 mM NaCl, cOmplete Protease Inhibitor Cocktail Tablets, followed by freeze-thaw.
- Mammalian extracts: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% Nonidet P40, complete Protease Inhibitor Cocktail Tablets.
- Other cell lysis buffers may be more appropriate for individual applications. In general, to obtain optimal performance of the affinity matrix:
- Use protease inhibitors to reduce proteolytic activity. Use complete Protease Inhibitor Cocktail Tablets for most applications.
- Limit detergent to the lowest concentration levels necessary to obtain adequate cell lysis.
Working concentration: Working concentration of conjugate depends on application and substrate
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 100 ng/ml
- ELISA: 100 ng/ml
- Western blot: 100 ng/ml
재구성
Add 1 ml double-distilled water to a final concentration of 50 μg/ml.
Rehydrate for 10 minutes prior to use.
Rehydrate for 10 minutes prior to use.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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