E6779

EZview^™ Red Anti-HA Affinity Gel

• 동의어(들): Anti-HA
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 유통기한: 1 yr at 2‑8 °C
• Quality Level: 200
• 분석물 화학적 분류: proteins (hemagglutinin)
• 기술: affinity chromatography: suitable; immunoprecipitation (IP): suitable
• 기질: crosslinked agarose
• 용량: ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)
• 저장 온도: 2-8°C

설명

일반 설명

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

특이성

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

애플리케이션

EZview^™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.

Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

특징 및 장점

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

기타 정보

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

물리적 형태

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

법적 정보

EZview is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable