추천 제품
유통기한
1 yr at 2‑8 °C
Quality Level
분석물 화학적 분류
proteins (c-Myc)
기술
affinity chromatography: suitable
immunoprecipitation (IP): suitable
용량
~0.05 mg/mL binding capacity
저장 온도
2-8°C
일반 설명
EZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.
애플리케이션
EZview® Red Anti-c-Myc Affinity Gel is suitable for:
- SUMOylation assay
- Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
특징 및 장점
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
기타 정보
The affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein.
물리적 형태
1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservative
법적 정보
EZview is a trademark of Sigma-Aldrich Co. LLC
SabreLite is a registered trademark of Pelican Products, Inc.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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