C3956

Anti-c-Myc antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Myc Tag Polyclonal Antibody, c-Myc Antibody
• MDL number: MFCD00239651
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 종 반응성: human
• 농도: ~0.5 mg/mL
• 기술: immunocytochemistry: 5-10 μg/mL; immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts; indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells; microarray: suitable; western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.
• UniProt 수납 번호: P01106
• 저장 온도: −20°C
• 유전자 정보: human ... MYC(4609)

설명

일반 설명

Anti-c-Myc antibody is used for the detection of the c-Myc tag sequence on c-Myc tagged fusion proteins.This polyclonal antibody is produced in rabbit and recognizes the c-Myc sequence at the N-terminus and C-terminus. C3956 is an Affinity purified antibody, increasing sensitivity in most applications.

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. It is located on chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.

특이성

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

면역원

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

애플리케이션

Anti-c-Myc antibody has been used in western blotting and immunofluorescence.

Antibody is recommended for use in immunoblotting, immunoprecipitation and immunofluorescence.
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생화학적/생리학적 작용

MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP). The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as transcriptional regulator of gene expression.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)