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Merck
모든 사진(1)

Key Documents

06-549

Sigma-Aldrich

Anti-Myc Tag Antibody

Upstate®, from rabbit

동의어(들):

Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human

포장

antibody small pack of 25 μg

제조업체/상표

Upstate®

기술

immunocytochemistry: suitable
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

일반 설명

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

특이성

Other species cross-reactivity not tested.
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.

면역원

Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

애플리케이션

Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.
Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

품질

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

표적 설명

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.

물리적 형태

Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.

분석 메모

Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Mxi1-SRalpha: a novel Mxi1 isoform with enhanced transcriptional repression potential.
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Development null
V M Draviam et al.
The Journal of cell biology, 152(5), 945-958 (2001-03-10)
In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization
K Ojima et al.
The Journal of cell biology, 150(3), 553-566 (2000-08-10)
To learn how nebulin functions in the assembly and maintenance of I-Z-I bands, MYC- and GFP- tagged nebulin fragments were expressed in primary cultured skeletal myotubes. Their sites of incorporation were visualized by double staining with anti-MYC, antibodies to myofibrillar

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