추천 제품
제품명
Anti-Myc Tag Antibody, clone 4A6, clone 4A6, Upstate®, from mouse
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
4A6, monoclonal
종 반응성
human
포장
antibody small pack of 25 μg
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... MYC(4609)
일반 설명
Identification of the target epitope is important for characterization of a monoclonal antibodies. Epitope tags are short, linear antibody recognition sequences that enable detection of tagged fusion proteins by antibodies. Over the years, a wide range of anti-tag antibodies have been developed and each one of these offer unique advantages and disadvantages. Epitope tags can undergo post-translational modifications, such as phosphorylation and glycosylation in cells and cause an increase in the molecular mass of the fused protein, which can result in changes in gel mobility. Epitope tags of smaller number of amino acid residues are advantageous in that they exhibit minimal side effects on structure and function of fused target protein. A Myc tag can be used in many different immunoassays and is useful when there is no antibody against the protein of interest. Adding a Myc-tag permits the detection of such proteins with an antibody against the Myc epitope. Clone 4A6 is an antibody that recognizes ten amino acid sequence EQKLISEEDL (Glu-Gln-Lys-Leu-Iso-Ser-Glu-Glu-Asp-Leu) corresponding to the C-terminal region of human c-Myc protein (UniProt: P01106). Unlike other clones whose performance may be affected when Myc tag sequence is followed by smaller neutral amino acids, Clone 4A6 offers much more consistent detection of Myc-tagged proteins irrespective of any changes in neighboring amino acid sequences. (Ref. Schuchner, S., et al. (2020). Sci. Signal. 13(616); eaax9730; Charest-Morin, X., et al. (2013). Front. Pharmacol. 4: 122).
특이성
Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
면역원
peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
애플리케이션
Anti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
품질
routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430)
표적 설명
Predicted MW for human Myc: 49 kDa
물리적 형태
Format: Purified
Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Protein G Purified
저장 및 안정성
Maintain at 2 to 8°C for 1 year from date of shipment.
분석 메모
Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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