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Merck
모든 사진(1)

주요 문서

G5166

Sigma-Aldrich

PNGase F from Elizabethkingia miricola

buffered aqueous solution

동의어(들):

N-Glycosidase F, Peptide N-glycosidase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32

생물학적 소스

bacterial (Elizabethkingia miricola)

Quality Level

결합

(N-linked)

양식

buffered aqueous solution

특이 활성도

≥20000 units/mg protein

분자량

36 kDa

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

애플리케이션

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.
Used to deglycosylate protein.

생화학적/생리학적 작용

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

단위 정의

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

물리적 형태

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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