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Merck
모든 사진(1)

문서

G8529

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

동의어(들):

G-6-P-DH

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

재조합

expressed in E. coli

Quality Level

형태

lyophilized powder

특이 활성도

≥550 units/mg protein (biuret)

구성

Protein, 10-40% biuret

응용 분야

agriculture

외래 활성

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

저장 온도

2-8°C

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애플리케이션

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

  • in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
  • to study the starch time course using Nicotiana tabacum leaves
  • to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
  • to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

생화학적/생리학적 작용

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.

단위 정의

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

물리적 형태

Lyophilized powder containing Ficoll and Tris buffer salts

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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문서 라이브러리 방문

Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Benjamin J Reisman et al.
Nature chemical biology, 18(4), 360-367 (2021-12-04)
Cancer cells have long been recognized to exhibit unique bioenergetic requirements. The apoptolidin family of glycomacrolides are distinguished by their selective cytotoxicity towards oncogene-transformed cells, yet their molecular mechanism remains uncertain. We used photoaffinity analogs of the apoptolidins to identify
Thomas Beneyton et al.
Nature communications, 9(1), 2391-2391 (2018-06-21)
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
A O Abolaji et al.
Human & experimental toxicology, 32(2), 206-215 (2012-11-17)
Among the artemisinin-based combination therapy (ACT) regimens, artemisinin derivative, artemether in combination with lumefantrine (artemether-lumefantrine, AL) has achieved excellent results in the fight against malarial scourge. In this study, we evaluated the toxic potential of these drugs at the therapeutic

프로토콜

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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