추천 제품
product name
Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
생물학적 소스
Porcine skin
Quality Level
무균
γ-irradiated
유형
Type A
제품 라인
BioXtra
형태
lyophilized powder
포장
pkg of 10 mg
기술
cell culture | mammalian: suitable
표면 범위
100‑200 μg/cm2
겔 강도
~300 g Bloom
solubility
soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)
배송 상태
ambient
저장 온도
room temp
유사한 제품을 찾으십니까? 방문 제품 비교 안내
애플리케이션
This product is recommended for use as a cell culture substratum at 100-200 μg/cm2. The optimal concentration does depend on cell type as well as the application and research objectives.
Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.
Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.
성분
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.
주의사항
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
제조 메모
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.
This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.
This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.
Storage Class Code
11 - Combustible Solids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
프로토콜
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
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