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Merck
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주요 문서

GE17-0041-01

Sephadex® G-50

Superfine, Cytiva, pack of 100 g

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
41115722
NACRES:
NA.56

특징

autoclavable In wet form (pH 7.0) at 120°C for 30 min

포장

pack of 100 g

제조업체/상표

Cytiva

파라미터

flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)

팽창

1 g swells to 9-11 mL

입자 크기

20-50 μm (dry)
20-80 μm (wet)

작동 범위

2-10

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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일반 설명

Sephadex® G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

특징 및 장점

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Small bead size for shorter diffusion distances
  • Smallest bead size in the Sephadex® G-50 range

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids


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시험 성적서(COA)

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문서 라이브러리 방문

Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure

관련 콘텐츠

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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