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Merck
모든 사진(2)

문서

S6022

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
23151817
NACRES:
NA.52

Grade

DNA grade
for molecular biology

Quality Level

제품 라인

BioReagent

형태

beads

팽창

1 g swells to 9-11 mL

비드(bead) 직경

50-150 μm

공극 크기

exclusion limit (20 bp dsDNA; (rA)20)

pH

2-10

외래 활성

DNase, RNase, none detected

저장 온도

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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일반 설명

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

애플리케이션

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

특징 및 장점

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

법적 정보

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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Pauly GB, et al.
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