H2625

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

• 동의어(들): Bovine hemoglobin, Hb, Methemoglobin
• CAS Number: 9008-02-0
• MDL number: MFCD00131282
• UNSPSC 코드: 12352202
• eCl@ss: 42030116
• NACRES: NA.61

속성

• 생물학적 소스: bovine blood
• 형태: substrate powder
• 분자량: M_r ~64500
• 기술: activity assay: suitable
• solubility: H₂O: soluble 20 mg/mL
• 적합성: suitable for protease substrate
• UniProt 수납 번호: P01966; P02070; P02081; P06642; P06643
• 저장 온도: 2-8°C
• 유전자 정보: cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)

설명

일반 설명

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

애플리케이션

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

생화학적/생리학적 작용

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.

Oxygen transporter. The Fe²⁺/Fe³⁺ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

주의사항

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

제조 메모

Prepared from washed, lysed and dialyzed erythrocytes.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)