추천 제품
product name
Cystatin C human, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
생물학적 소스
human
Quality Level
재조합
expressed in HEK 293 cells
분석
≥95% (SDS-PAGE)
형태
lyophilized powder
효능
≤5 μM IC50
품질
endotoxin tested
분자량
dimer 12-13 kDa (non-glycosylated)
포장
pkg of 10 μg
저장 조건
avoid repeated freeze/thaw cycles
기술
cell culture | mammalian: suitable
불순물
≤1 EU/mg
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
human ... CYTC(1471)
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일반 설명
HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.
애플리케이션
Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).
생화학적/생리학적 작용
Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.
물리적 형태
Lyophilized from a 0.2 μm filtered solution of 1×PBS
분석 메모
The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.
법적 정보
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Assessment of glomerular filtration rate in acute and chronic settings
National Kidney Foundation Primer on Kidney Diseases, 26-32 (2014)
M S N Murty et al.
Indian journal of nephrology, 23(3), 180-183 (2013-07-03)
In patients with acute kidney injury (AKI), serum creatinine level does not increase until moderate to severe reduction in glomerular filtration rate (GFR) occurs. Thus its use for estimating GFR in early AKI delays detection of kidney damage and making
Assessing kidney function
Chronic Renal Disease, 31-42 (2015)
Can-E Tang et al.
Journal of proteome research, 9(12), 6101-6111 (2010-10-05)
The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated secreted
Jean-Charles Lafarge et al.
Biochimie, 92(11), 1580-1586 (2010-04-27)
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