H9395

α-Hemolysin from Staphylococcus aureus

lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

• 동의어(들): α-Toxin
• CAS Number: 94716-94-6
• MDL number: MFCD00130489
• UNSPSC 코드: 12352202
• NACRES: NA.56

속성

• 생물학적 소스: Staphylococcus aureus
• Quality Level: 300
• 형태: lyophilized powder
• 특이 활성도: ≥10,000 units/mg protein
• 포함: sodium citrate buffer as balance
• 구성: Protein, ~60% Lowry
• solubility: H₂O: soluble 0.49-0.51 mg/mL
• UniProt 수납 번호: Q2G1X0
• 저장 온도: 2-8°C
• 유전자 정보: Staphylococcus aureus ... SAOUHSC_01121(3920722)

설명

일반 설명

α-Hemolysin, a pore-forming cytotoxin is an extracellular protein secreted by most strains of pathogenic Staphylococcus aureus. It is secreted as a water-soluble monomer and is a small β-barrel protein.

애플리케이션

α-Hemolysin from Staphylococcus aureus has been used:

• as a component of electrolyte solution for testing pore formation in lipid bilayer using electrophysiological measurements
• to test its osteogenesis suppressive effects in bone marrow stromal cells (BMSCs)
• in the preparation of α-hemolysin molecular imprinted polymer (MIP) for Biacore and surface plasmon resonance

α-Hemolysin was used in a study to test the efflux pump and haemolysin activity of Escherichia coli of dairy origin. It was also used to test its adaptation to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation.

생화학적/생리학적 작용

α-Hemolysin is selectively hemolytic and the monomeric form binds to a membrane and specific receptors are not required for binding. Upon binding to biological membranes and/or artificial membranes, self-oligomerization occurs, resulting in ring structures (hexameric aggregates) believed to represent transmembrane pores, which are permeable to ions and small metabolites. It has a marked preference for rabbit red blood cells. α-hemolysin stimulates cellular phospholipases and induces a Ca²⁺ influx. It leads to membrane disruption of the endothelial barrier and leakage of cytoplasmic components and osmotic lysis of the cells. α-hemolysin is implicated in the pathogenesis of sepsis.

포장

Package size based on protein content

단위 정의

One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319,H371
• 예방조치 성명서: P260 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P311
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2
• 표적 기관: Lungs,Blood
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)