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Merck
모든 사진(2)

주요 문서

L0881

Sigma-Aldrich

Lectin from Arachis hypogaea (peanut)

lyophilized powder, Affinity-purified

동의어(들):

PNA, Peanut agglutinin

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.32

양식

lyophilized powder

Quality Level

효능

<0.1 μg per mL agglutination activity

품질

Affinity-purified

불순물

salt, free

저장 온도

−20°C

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일반 설명

Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.

애플리케이션

Lectin from Arachis hypogaea (peanut) has been used:
  • to detect Thomson-Friedenreich antigen
  • as a component in PBS to study its effects on cell viability
  • to study its ability to block the binding site

생화학적/생리학적 작용

Lectin from Arachis hypogaea (peanut) possess specificity towards β -D-Gal(1-3)-D-galNAc residue of Thomson − Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

분석 메모

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Adhesion of plant roots to poly-L-lysine coated polypropylene substrates
Towler MJ and Weathers PJ
Journal of Biotechnology, 101(2), 147-155 (2003)
Expression of peanut agglutinin-binding mucin-type glycoprotein in human esophageal squamous cell carcinoma as a marker
Kannan S, et al.
Molecular Cancer, 2(1), 38-38 (2003)
Alejandro J Cagnoni et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 11), 1080-1091 (2020-11-03)
Carbohydrate-lectin interactions are involved in important cellular recognition processes, including viral and bacterial infections, inflammation and tumor metastasis. Hence, structural studies of lectin-synthetic glycan complexes are essential for understanding lectin-recognition processes and for the further design of promising chemotherapeutics that
In vitro and in vivo antitumor effects of Peanut agglutinin through induction of apoptotic and autophagic cell death
Mukhopadhyay S, et al.
Food And Chemical Toxicology, 64, 369-377 (2014)
Lectin binding and effects in culture on human cancer and non-cancer cell lines: examination of issues of interest in drug design strategies
Petrossian K, et al.
Acta Histochemica, 109(6), 491-500 (2007)

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